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Sangon Biotech small interfering rna sirna
Small Interfering Rna Sirna, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/small+interfering+(sirnas)/interfering+rnas+small/pm42242505-77-0-10
Average 86 stars, based on 1 article reviews
small interfering rna sirna - by Bioz Stars, 2026-11
86/100 stars

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Article Title: Geniposide enhances cisplatin sensitivity in non-small cell lung cancer through PUMA-mediated apoptotic pathway.
Article Snippet: Background: Cisplatin-based chemotherapy remains the standard treatment for advanced non-small cell lung cancer (NSCLC); however, its efficacy is constrained by acquired drug resistance and dose-limiting toxicity.. Dysregulated apoptosis is recognized as a key mechanism contributing to cisplatin resistance in NSCLC.. Geniposide, an active component of traditional Chinese medicine, has demonstrated broad-spectrum antitumor activity; however, its role in cisplatin resistance remains unclear.

Knockdown:

Article Title: Multiomics Reveals IL-17 Drives Epithelial Keratinization and Proliferation via EHF in Odontogenic Keratocysts
Article Snippet: HOKs (Catalog No. 2610, ScienCell, Carlsbad, CA, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (HyClone, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA) at 37 °C in a 5% CO 2 humidified incubator. .. EHF knockdown was achieved by transfecting cells with small interfering RNAs (siRNAs) designed via siDirect 2 (EHF-si1, si2, si3) [ , ] or a non-targeting control (CON-si) (Sangon Biotech, Shanghai, China) ( ). .. Transfections were performed using LipofectamineTM 3000 Transfection Reagent (Thermo Fisher Scientific, Waltham, MA, USA) at a final siRNA concentration of 100 nM.

Article Title: Matrix metalloproteinase 2 governs extracellular matrix remodeling during ovarian development in a viviparous teleost Sebastes schlegelii (Hilgendorf, 1880).
Article Snippet: .. Ovarian tissue block culture and mmp2 knockdown both in vitro and in vivo of S. schlegelii According to the coding sequences (CDS) characteristics of mmp2, three specific siRNAs targeting mmp2 (mmp2-801-siRNA, mmp2-1731-siRNA, mmp2-1872siRNA) and a shRNA plasmid targeting mmp2 (mmp2-1872-shRNA) were designed and synthesized by Sangon Biotech, with the detailed sequences provided in Table S2. ..

Article Title: Multiomics Reveals IL-17 Drives Epithelial Keratinization and Proliferation via EHF in Odontogenic Keratocysts.
Article Snippet: HOKs (Catalog No. 2610, ScienCell, Carlsbad, CA, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (HyClone, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA) at 37 ◦C in a 5% CO2 humidified incubator. .. EHF knockdown was achieved by transfecting cells with small interfering RNAs (siRNAs) designed via siDirect 2 (EHF-si1, si2, si3) [38,39] or a non-targeting control (CON-si) (Sangon Biotech, Shanghai, China) (Supplementary Table S1). .. Transfections were performed using LipofectamineTM 3000 Transfection Reagent (Thermo Fisher Scientific, Waltham, MA, USA) at a final siRNA concentration of 100 nM.

Control:

Article Title: Multiomics Reveals IL-17 Drives Epithelial Keratinization and Proliferation via EHF in Odontogenic Keratocysts
Article Snippet: HOKs (Catalog No. 2610, ScienCell, Carlsbad, CA, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (HyClone, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA) at 37 °C in a 5% CO 2 humidified incubator. .. EHF knockdown was achieved by transfecting cells with small interfering RNAs (siRNAs) designed via siDirect 2 (EHF-si1, si2, si3) [ , ] or a non-targeting control (CON-si) (Sangon Biotech, Shanghai, China) ( ). .. Transfections were performed using LipofectamineTM 3000 Transfection Reagent (Thermo Fisher Scientific, Waltham, MA, USA) at a final siRNA concentration of 100 nM.

Article Title: Multiomics Reveals IL-17 Drives Epithelial Keratinization and Proliferation via EHF in Odontogenic Keratocysts.
Article Snippet: HOKs (Catalog No. 2610, ScienCell, Carlsbad, CA, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (HyClone, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA) at 37 ◦C in a 5% CO2 humidified incubator. .. EHF knockdown was achieved by transfecting cells with small interfering RNAs (siRNAs) designed via siDirect 2 (EHF-si1, si2, si3) [38,39] or a non-targeting control (CON-si) (Sangon Biotech, Shanghai, China) (Supplementary Table S1). .. Transfections were performed using LipofectamineTM 3000 Transfection Reagent (Thermo Fisher Scientific, Waltham, MA, USA) at a final siRNA concentration of 100 nM.

Generated:

Article Title: Single-cell profiling of tumor lineage plasticity and the immune microenvironment in transformed small cell lung cancer.
Article Snippet: .. Kaplan-Meier survival curves were generated using the R function “ggsurvplot”. siRNA transfection siRNAs targeting interferon alpha and beta receptor subunit 1 (IFNAR1) gene were purchased from Sangon Biotech (Shanghai, China). ..

Transfection:

Article Title: Single-cell profiling of tumor lineage plasticity and the immune microenvironment in transformed small cell lung cancer.
Article Snippet: .. Kaplan-Meier survival curves were generated using the R function “ggsurvplot”. siRNA transfection siRNAs targeting interferon alpha and beta receptor subunit 1 (IFNAR1) gene were purchased from Sangon Biotech (Shanghai, China). ..

Synthesized:

Article Title: Integrative machine learning models reveal immune and metabolic signatures predictive of colorectal cancer prognosis.
Article Snippet: This study utilized one human normal colon epithelial cell line (NCM460) and four human CRC cell lines (HT29, HCT116, SW480, and SW620), all obtained from the American Type Culture Collection (ATCC). .. IL20RB gene silencing was performed in the HT29 and SW620 CRC cell lines using specific small interfering RNAs (siRNAs) synthesized by Sangon Biotech (Shanghai, China). ..

Article Title: Matrix metalloproteinase 2 governs extracellular matrix remodeling during ovarian development in a viviparous teleost Sebastes schlegelii (Hilgendorf, 1880).
Article Snippet: .. Ovarian tissue block culture and mmp2 knockdown both in vitro and in vivo of S. schlegelii According to the coding sequences (CDS) characteristics of mmp2, three specific siRNAs targeting mmp2 (mmp2-801-siRNA, mmp2-1731-siRNA, mmp2-1872siRNA) and a shRNA plasmid targeting mmp2 (mmp2-1872-shRNA) were designed and synthesized by Sangon Biotech, with the detailed sequences provided in Table S2. ..

Article Title: Electroacupuncture combined with rTMS promotes neuronal regeneration via DNMT1-mediated PI3K-AKT pathway in cerebral palsy model
Article Snippet: .. Three specific small interfering RNAs (siRNAs) targeting the rat Dnmt1 transcript were designed based on the sequence NM_053354.3 and synthesized by Sangon Biotech (Shanghai, China). ..

Article Title: Expression and Transcriptional Response of sirt1 in Yellow Catfish (Pelteobagrus fulvidraco) Under Acute Hypoxia Stress.
Article Snippet: .. The roles of Sirt1 in other high-expression tissues such as the brain and heart under hypoxic conditions warrant further investigation in future studies. siRNA design and screening: Three siRNAs (siRNA-1, siRNA-2 and siRNA-3) targeting different regions of the yellow catfish sirt1 mRNA (GenBank accession no. NC_062521.1) were designed and synthesized by Sangon Biotech (Shanghai) Co., Ltd. (Shanghai, China). ..

Blocking Assay:

Article Title: Matrix metalloproteinase 2 governs extracellular matrix remodeling during ovarian development in a viviparous teleost Sebastes schlegelii (Hilgendorf, 1880).
Article Snippet: .. Ovarian tissue block culture and mmp2 knockdown both in vitro and in vivo of S. schlegelii According to the coding sequences (CDS) characteristics of mmp2, three specific siRNAs targeting mmp2 (mmp2-801-siRNA, mmp2-1731-siRNA, mmp2-1872siRNA) and a shRNA plasmid targeting mmp2 (mmp2-1872-shRNA) were designed and synthesized by Sangon Biotech, with the detailed sequences provided in Table S2. ..

In Vitro:

Article Title: Matrix metalloproteinase 2 governs extracellular matrix remodeling during ovarian development in a viviparous teleost Sebastes schlegelii (Hilgendorf, 1880).
Article Snippet: .. Ovarian tissue block culture and mmp2 knockdown both in vitro and in vivo of S. schlegelii According to the coding sequences (CDS) characteristics of mmp2, three specific siRNAs targeting mmp2 (mmp2-801-siRNA, mmp2-1731-siRNA, mmp2-1872siRNA) and a shRNA plasmid targeting mmp2 (mmp2-1872-shRNA) were designed and synthesized by Sangon Biotech, with the detailed sequences provided in Table S2. ..

In Vivo:

Article Title: Matrix metalloproteinase 2 governs extracellular matrix remodeling during ovarian development in a viviparous teleost Sebastes schlegelii (Hilgendorf, 1880).
Article Snippet: .. Ovarian tissue block culture and mmp2 knockdown both in vitro and in vivo of S. schlegelii According to the coding sequences (CDS) characteristics of mmp2, three specific siRNAs targeting mmp2 (mmp2-801-siRNA, mmp2-1731-siRNA, mmp2-1872siRNA) and a shRNA plasmid targeting mmp2 (mmp2-1872-shRNA) were designed and synthesized by Sangon Biotech, with the detailed sequences provided in Table S2. ..

shRNA:

Article Title: Matrix metalloproteinase 2 governs extracellular matrix remodeling during ovarian development in a viviparous teleost Sebastes schlegelii (Hilgendorf, 1880).
Article Snippet: .. Ovarian tissue block culture and mmp2 knockdown both in vitro and in vivo of S. schlegelii According to the coding sequences (CDS) characteristics of mmp2, three specific siRNAs targeting mmp2 (mmp2-801-siRNA, mmp2-1731-siRNA, mmp2-1872siRNA) and a shRNA plasmid targeting mmp2 (mmp2-1872-shRNA) were designed and synthesized by Sangon Biotech, with the detailed sequences provided in Table S2. ..

Plasmid Preparation:

Article Title: Matrix metalloproteinase 2 governs extracellular matrix remodeling during ovarian development in a viviparous teleost Sebastes schlegelii (Hilgendorf, 1880).
Article Snippet: .. Ovarian tissue block culture and mmp2 knockdown both in vitro and in vivo of S. schlegelii According to the coding sequences (CDS) characteristics of mmp2, three specific siRNAs targeting mmp2 (mmp2-801-siRNA, mmp2-1731-siRNA, mmp2-1872siRNA) and a shRNA plasmid targeting mmp2 (mmp2-1872-shRNA) were designed and synthesized by Sangon Biotech, with the detailed sequences provided in Table S2. ..

Sequencing:

Article Title: Electroacupuncture combined with rTMS promotes neuronal regeneration via DNMT1-mediated PI3K-AKT pathway in cerebral palsy model
Article Snippet: .. Three specific small interfering RNAs (siRNAs) targeting the rat Dnmt1 transcript were designed based on the sequence NM_053354.3 and synthesized by Sangon Biotech (Shanghai, China). ..



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